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Anti-IL-6 antibody for autoimmune Rheumatoid Arthritis

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Association of AFF3 Gene Polymorphism rs10865035 with Rheumatoid Arthritis: A Population-Based Case-Control Study on a Pakistani Cohort

We previously reported that penta-acetyl geniposide ((Ac)5GP, an active derivative of geniposide) showed anti-arthritic effect on adjuvant-induced arthritis (AIA) rats by promoting the apoptosis of AIA fibroblast-like synoviocyte (FLS). This study aimed to demonstrate the effects of (Ac)5GP on migration, invasion, and inflammation of TNF-α-stimulated rheumatoid arthritis (RA) FLS (MH7A cell) and to explore the involved mechanisms. MTT assay was used to determine the applied non-cytotoxic doses of (Ac)5GP (12.5, 25, 50 μM) in vitro. Results of wound-healing, transwell, and phalloidin staining assays indicated that (Ac)5GP reduced the migration, invasion, and F-actin cytoskeletal reorganization of TNF-α-stimulated MH7A. Results of ELISA and western blot assays confirmed that (Ac)5GP reduced TNF-α-induced production of pro-inflammatory cytokines (like IL-1β, IL-6, IL-8) and matrix metalloproteinases (MMPs, such as MMP-2 and MMP-9). Moreover, (Ac)5GP inhibited TNF-α-induced activation of Wnt/β-catenin pathway, evidenced by reducing the protein levels of Wnt1, p-GSK-3β (Ser9), and β-catenin and preventing β-catenin nuclear translocation. Importantly, the combination of XAV939 (an inhibitor of Wnt/β-catenin) promoted the actions of (Ac)5GP on TNF-α-induced migration, invasion, and inflammation, further revealing the involvement of Wnt/β-catenin pathway underlying the therapeutic effects of (Ac)5GP on TNF-α-stimulated MH7A. In vivo, (Ac)5GP relieved the progression and severity of rat collagen-induced arthritis, related to reducing the levels of IL-1β, IL-6, IL-8, MMP-2, and MMP-9 as well as inhibiting Wnt/β-catenin pathway in synovial tissues. Collectively, (Ac)5GP could suppress TNF-α-induced migration, invasion, and inflammation in RA FLS involving Wnt/β-catenin pathway and (Ac)5GP might be as a candidate agent for RA treatment.
June 30, 2021 Jon 0 Comments

Rheumatoid arthritis (RA) is likely one of the advanced ailments with the involvement of the genetic in addition to environmental elements in its onset and severity. Completely different genome-wide affiliation and candidate gene research have proven the function of a number of genetic variants in a number of loci/genes with ethnical and geographical variations. This …

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Categories

  • colorimetric
  • ELISA test
  • high
  • Mouse
  • Rat
  • Standards

Recent Posts

  • Investigating Glycosylation Patterns with Mass Spectrometric Analysis: A transformative development in Bioconjugation Techniques and Drug Discovery
  • Harnessing the capability of Glycan-Protein Associations: A cutting-edge technique in Vaccine Engineering
  • Utilizing the efficiency of Glycan-Protein Associations: A new approach in Vaccine Development
  • Glycan Modification and Its Impact on Monoclonal Antibody Therapeutics: Optimizing Effectiveness Through Glycan Engineering
  • Sources of Lab reagents

Tags

biology cells notes biology cells parts biology cells pdf biology cells practice test biology cells questions biology cells quiz biology cells quiz 3 biology cells quizlet biology cells review biology cells revision flashcards gcse biology cells revision notes gcse biology cells study biology cells study guide biology cells test biology cells unit biology cells unit test biology cells unit test answers biology cells worksheet biology cells worksheets answers biology cells worksheets pdf cannabis assay kits creatinine assay kits enzymes for digestion of lipids enzymes for dogs enzymes for dogs joints enzymes for drain enzymes for drain pipes enzymes for fat enzymes for gluten enzymes for heart exosomes and chf gelson’s rancho mirage gelson’s thousand oaks gelson’s weekly flyer gelsosomo’s crown point gelsosomo’s pizza gelsosomo’s pizza chesterton gelston house east haddam ct gelstx gelsyn-3 gelsyn-3 injection gelsyn injection isotopes and ions mineral assay kits msd assay kits
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